Effects of water and substratum nutrient supplies on lotic periphyton growth: An integrated bioassay.

Abstract:   Effects of substratum and water nutrient purterbations on periphyton growth were assessed in a nutrient-poor stream by combining a substratum enrichment technique with a flow-through bioassay system.  Periphyton growth (chlorophyll a, total biovolume) responded to combined influences of water and substratum enrichment in an additive manner when both compartments were amended with N and P to yield an optimal ratio (N:P ~16:1). When NO3-N was added to the substratum and PO4-P to the water, algal growth response was synergistic. Analysis of the vertical distributions of P fractions in cores taken from nutrient-diffusing substrata indicates that attached microorganisms mediate P release from underlying substrata, acting as a filter or temporary sink.  Nutrient-diffusing substrata are useful detectors of limiting nutrients in aquatic systems; however, their function and application differ from water enrichment assays where nutrients are added at a constant rate.  Differences are partially attributed to spatial and temporal variability of nutrient release and the strictly localized influence of substratum flora on ambient water chemistry.


Pringle, C.M. 1987. Effects of water and substratum nutrient supplies on lotic periphyton growth: An integrated bioassay. Canadian Journal of Fisheries and Aquatic Sciences 44: 619-629.

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